RMI Micro-Imaging Core
Overview
The RMI Micro-Imaging Core Facility supports research through high resolution confocal imaging of cells and tissues. The facility is located in Room 306 on the third floor of the newly renovated Steven Spielberg Building, and is under the direction of Terrence Town, PhD and operated by Altan Rentsendorj, PhD. The core provides RMI users with a Nikon C1si Eclipse confocal instrument that is equipped with four laser-lines to allow simultaneous visualization of up to 5 florophores in standard mode and up to 32 colors using the attached spectral detector. The instrument allows for effective observation of expression-multiplexed specimens stained with CFP, GFP, YFP, DsRed and similar reagents, as well as imaging of localized proteins and Fluorescence Resonance Energy Transfer (FRET) observation. The instrument can image fixed cells and tissues and live cells for short (~30 minutes) duration, and acquires images with a resolution of up to 2048 x 2048 pixels. Also featured is Nikon’s Perfect Focus System™ (PFS), which uses infra-red light to maintain focus on specimens. In summary, the core provides state of the art confocal microscopy to the RMI user-base.
Contact information
Director: Terrence Town, PhD
E-mail: terrence.town@cshs.org
Phone: 310-248-8581
Coordinator: Tara Weitz
E-mail: weitzt@cshs.org
