Instrumentation
1. QC: Agilent Bioanalyzer, Qubit, E-gel, qPCR
2. LIMS – Laboratory information system for organization, tracking, and access of genomic data.
3. 2 Microarray platforms - Illumina iScan and Affymetrix
4. 4 New qPCR platforms for gene expression (single cell), high throughput validation (2K-10K Data)
a. Tecan (automated fluidics for Viia7), Viia7, ABI Open Array, Fluidigm
5. Sequencing
a. Illumina GAIIx, Paired End module, and cBot
6. Computation
a. Developmental Cluster -4 compute nodes (4 quad core chips, 48 GB each)
b. Production Cluster
i. 4 compute nodes (4 quad cores, 48 GB each)
ii. 6 compute nodes (6 quad cores, 96 GB each)
iii. 400 compute nodes (2 cores, 2GB each)
7. Data Access and Analysis
a. Remotely available
i. VM1 – LIMs web interface
ii. VM2 – CLC bioinformatics, 6 licenses
iii. 24 GB RAM, Quad core
b. Locally available in Bioinformatics suite
i. 27 inch MAC i7 quad core 8GB ram 1.5 TB
ii. 12 GB Quad core Workstation 2 TB
Nanodrop
Industrial quantitation for large number of DNA or RNA samples.
Agilent Bioanalyzer
Industry standard to assess quality of RNA in samples with as little as 250 picograms.
Affymetrix Gene Expression Profiling
The industry standard of mRNA and miRNA gene expression profiling.
Tecan Freedom EVO 100
Robotic fluid handling automation for large scale qRT-PCR and sample automation.
Illumina iScan
Inexpensive high throughput gene expression profiling.
