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What are induced pluripotent stem cells (iPSCs)?
Induced pluripotent stem cells are adult cells that have been genetically reprogrammed to an embryonic stem cell-like state. This is achieved by forcing the cells to express genes and factors important for maintaining the defining properties of embryonic stem cells.
What are the utilities of iPSCs?
Human iPSCs are unprecedented tools for the study of human development, cell fate programming, tissue regeneration and drug development. They are also used to produce many human system cell types for studying human development and other biological investigations.
What services does the iPSC Core offer?
The RMI iPSC core offers:
- Custom iPSC generation
- Banking and expansion of iPSC lines
- iPSC characterization
For more information on any of these services please visit Services.
How does the iPSC Core generate iPSCs?
The RMI iPSC Core generates '"footprint free" iPSCs by transfecting parent cells with non-integrating oriP/EBNA1 plasmids that rely on episomal expression of reprogramming factors.
What tissues can the iPSC Core reprogram?
The RMI iPSC Core can reprogram various human somatic tissues including fibroblast, lymphoblastoid cell lines, corneal epithelial cells, primary keratocytes, adipose stem cells, mammary epithelial cells and whole blood.
How does the iPSC Core culture iPSCs?
Currently the RMI iPSC Core cultures all iPSC and hESC lines by feeder-free method using BD Matrigel substrate and mTeSR1 or E8. Protocols for feeder-free culture can be found in Protocols.
Do you provide mouse embryonic feeders for feeder-based culture?
The core provides mitotically inactivated MEFs generated in-house and treated with mitomycin-C that have been quality control tested on existing ES and iPSC lines. Vials can be purchased on the Cell Lines page.
Can I request cell lines from the iPSC Core?
Yes, visit the Cell Lines page for a list of available cells lines and directions on how to order cells from the core.
Can I order supplies from the iPSC Core?
Yes, cell culture reagents and supplies can be ordered from the core. A list of available reagents and pricing information is available at Ordering Supplies.
How can I contact the RMI iPSC Core?
You can find all of the iPSC Core contact information on our Contact page.