Services and Prices
Contact the RMI iPSC Core for a quote on any of these services.
Custom iPSC Generation
The RMI iPSC Core offers custom iPSC generation. We have experience in reprogramming a variety of somatic cells, including fibroblast, lymphoblastoids, corneal epithelial cells, primary keratocytes, adipose stem cells and mammary epithelial cells. We are actively working to reprogram blood cells. For each patient iPSC line, 3 clones will be expanded, banked and thoroughly characterized by the core prior to distribution under feeder-free conditions. Each of the cell lines will be accompanied by a rigorous Certificate of Analysis to ensure pluripotency, normal karyotype, and embryoid body formation, as well as a variety of services related to the characterization of the pluripotent state.
Pluripotent Stem Cell (PSC) Characterization
The RMI iPSC Core offers a variety of assays to ensure the quality and pluripotency of iPSC lines. PSC characterization is included in the custom iPSC generation service, or separately, as needed. These assays include:
- Mycoplasma testing
- Alkaline phosphatase staining
- Immunocytochemistry (ICC) for pluripotency markers
- G-Band karyotyping
- Pluripotent gene expression (qRT-PCR)
- OCT4/SSEA-4 analysis through FACS
- Embryoid body (EB) formation and analysis
- Illumina gene-chip expression and bioinformatics assay (PluriTest)
- Genomic DNA PCR (to confirm lack of plasmid integration)
- Southern blot analysis (optional)
- Teratomaformation (optional)
Expansion and Banking of iPSC Lines
As part of the custom iPSC generation service, the core will expand and bank 3 fully characterized iPSC clones per cell line. This service is also available to iPSC and ESC lines generated outside of Cedars-Sinai.
The RMI iPSC Core has a variety of control and diseased iPSC lines available for research. More information and a list of cell lines, are available on the Stem Cell Lines page.
Here you can find required forms needed for RMI iPSC Core services.