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Services and Prices
Contact the RMI iPSC Core for a quote on any of these services.
Custom iPSC Generation Package
Includes generation of 3 clonal iPSC lines per patient sample and most pluripotency characterization services*.
|External (Outside CSMC)||$12,000|
*See table below for characterization services included package (*)
Source tissues can include: fibroblast (skin), MSCs, adipose cells, various and stromal epithelial cell types, lymphoblastoid cells lines (LCLs or EBV B-cells) and fresh whole blood. The fresh blood must be collected in CPT tubes and centrifuged according to manufacture's directions. All reprogramming is preformed using non-integrating techniques in defined media (serum and KSR-free) and feeder-free conditions (no MEFs). Cost for each patient line includes the expansion and banking of 3 throughly characterized clonal lines under feeder-free conditions. Each of the cell lines will be accompanied by a rigorous Certificate of Analysis (COA) to ensure pluripotency, normal karyotype, embryoid body formation and a variety of services related to the characterization of the pluripotent state (see below).
Pluripotent Stem Cell (PSC) Quality Control and Characterization Services
The RMI iPSC Core offers a variety of assays to ensure the quality and pluripotency of PSC lines. PSC characterization is included in the custom iPSC generation service, or separately as needed.
|Service||Requirements||Price/ iPSC Line (1 clone)||Price/ iPSC Line (3 clones)|
|Mycoplasma Testing*||Please provide the Core with at least 1ml of 2 day old conditioned media. The Core regularly performs mycoplasma testing once a month. Contact the Core for the next available mycoplasma testing date.||$17.00||$50.00|
|Alkaline Phosphatase (AP) Staining*||Please provide the Core with 1 well of 50-60% confluent cells.||$40.00||$110.00|
|Immunocytochemistry (ICC) staining images* SSEA4 + OCT4 |
TRA-1-60 + NANOG
TRA-1-81 + SOX2
|Please proved the Core with 1 well of good quality cells that are at least 50-60% confluent.||$80.00||$225.00|
|OCT 3/4 + NANOG (or SSEA4) Imaging/Flow Cytometry*||6 samples, minimum, are required for running this assay. Price may be split between multiple investigators and Core can help coordinate. Please provide the Core with 1 confluent well of each sample.||$120.00||$330.00|
|G-Band Karyotype*||Please provide the Core with 2- 80% confluent wells.||$450.00||$1250.00|
|PluriTest Assay*||Please provide the Core with 1-2- 80% confluent wells.||$320.00||$900.00|
|RT-qPCR for endogenous/exogenous pluripotency gene expression*||Please provide the Core with 1-2 wells of good quality cells that are at least 80% confluent.||$100.00||$280.00|
|Embryoid Body (EB) formation, RT-PCR gene expression for 3 lineages.*||Please provide the Core with a 70-80% confluent 6-well plate of cells.||$340.00||$950.00|
|Teratoma formation||Price per clone. Contact the Core for more information regarding teratoma formation.||$2500.00||$6500.00|
|hPSC Scorecard Assay*||Price is per clone. Please provide the Core with a 70-80% confluent 6-well plate of cells. We will generate EBs and also test parent iPSCs. This assay is done in pair of iPSCs + EBs generated from the iPSCs for 10-18 days. Price reflected is for the pair.||$600.00||$1500.00|
|EBNA1 gDNA PCR*||Confirm lack of exogenous plasmid presence. Price is per clone.||$200.00||$550.00|
|Southern Blot Analysis||Confirm lack of plasmid integration.||$300.00||$850.00|
Services listed in BOLD are not included on the standard iPSC Generation Package. These services can be requested at an additional charge using the rates described above.
Bio-Banking and PSC Thawing
As part of the custom iPSC generation service, the core will expand and bank 3 fully characterized iPSC clones per cell line. This service is also available to iPSC and ESC lines generated outside of Cedars-Sinai.
|Cell Line Cryopreservation||Guaranteed cryopreservation of high quality, mycoplasma negative cells (6 vials total-feeder free).||Please provide the Core with 1 well of starter cells.||$150.00|
|PSC Thaw||The Core will thaw 1 vial of PSCs into 1 Matrigel coated well of a 6-well plate and maintain cells for 1 week.||Please provide the Core with 1 vial of cryopreserved cells.||$50.00|
|PSC Thaw and Expansion||The Core will thaw 1 vial of PSCs into 1 Matrigel coated well of a 6-well plate and expand cells into a full 6-well plate over the course of 2 weeks.||Please provide the Core with 1 vial of cryopreserved cells.||$150.00|
|PSC Research Grade Bio-Bank||The Core will generate a research grade bank of multiple cryopreserved vials of PSCs.||Karyotype will be performed prior to initiating banking and at the time of creating a research grade lot.||Contact Us|
Imaging Services - Image Express Micro, Molecular Devices
|Image Express Micro||Independent acquisition and analysis.||User must be trained.||$75.00/hr|
|Image Express Micro||Staff assisted acquisition and analysis.||User must be trained.||$120.00/hr|
To see a complete list of available cell lines, please visit our Stem Cell Lines page.
|hiPSCs or hESCs||Cryovial||Frozen||$650.00|
|hiPSCs or hESCs*||6-well plate||Live Culture||$120.00 ($20.00/well) + $650.00 (cost of vial) = $770.00|
|Reporter cell lines (iPSCs or ESCs) Nuclear GFP in AAVS1 safe harbor locus||6-well plate||Live Culture||$120.00|
|Reporter cell lines (iPSCs or ESCs) Nuclear GFP in AAVS1 safe harbor locus||Cryovial||Frozen||$700.00|
PSC qualified and mitomycin-C treated
|MEF vial suitable for 3 plates||Cryovial - 5x10^6 cells||Frozen||$70.00|
|MEF vial suitable for 6 plates||Cryovial - 10x10^6 cells||Frozen||$130.00|
|Mouse Embryonic Fibroblasts (MEF)||6-well plate||Live culture||$30.00|
|Mouse Embryonic Fibroblasts (MEF)||T75 flask||Live culture||$35.00|
*Shipping and Tax will be additional
Custom Mouse Embryonic Fibroblast (MEF) Generation
Contact the Core for custom generation of P1 and P2 non-mitomycin treated MEFs.
The iPSC Core trains graduate students, postdoctoral fellows and technicians in stem cell biology techniques. To schedule a training appointment, please contact the iPSC Core.