Services and Prices

Contact the RMI iPSC Core for a quote on any of these services.

 

Custom iPSC Generation Package

Includes generation of clonal iPSC lines for a patient sample provided by customer and complete1 or essential2 pluripotency characterization services. Please contact us for prices and IRB clearance if an iPSC line needs to be derived from fresh skin biopsy or a peripheral blood draw. For the packages with essential characterization suite, characterization data is provided 4 weeks after delivery of the iPSC lines.

SourcePackageCharacterization SuitePrice (Academic)
Fibroblasts3 clones at p12-p15Complete$12,000
2 clones at p12-p15Complete$10,000
1 clone at p12-p15Complete$8,000
3 clones at p5Essential$6,250
2 clones at p5Essential$5,250
1 clone at p5Essential$4,250
LCLs or peripheral blood3 clones at p12-p15Complete$12,500
2 clones at p12-p15Complete$10,500
1 clone at p12-p15Complete$8,500
3 clones at p5Essential$6,750
2 clones at p5Essential$5,750
1 clone at p5Essential$4,750

See table below for a description of the complete1 and essential2 characterization suites.

Source tissues can include: fibroblast (skin), MSCs, adipose cells, various and stromal epithelial cell types, lymphoblastoid cells lines (LCLs or EBV B-cells) and fresh whole blood. The fresh blood must be collected in CPT tubes and centrifuged according to manufacture's directions. All reprogramming is preformed using non-integrating techniques in defined media (serum and KSR-free) and feeder-free conditions (no MEFs). Cost for each patient line includes the expansion and banking of 3 throughly characterized clonal lines under feeder-free conditions. Each of the cell lines will be accompanied by a rigorous Certificate of Analysis (COA) to ensure pluripotency, normal karyotype, embryoid body formation and a variety of services related to the characterization of the pluripotent state (see below).

Prices listed above reflect academic pricing only. Please contact the iPSC Core for information on industry pricing.

Pluripotent Stem Cell (PSC) Quality Control and Characterization Services

The RMI iPSC Core offers a variety of assays to ensure the quality and pluripotency of PSC lines. PSC characterization is included in the custom iPSC generation service, or separately as needed. 

ServiceRequirementsPrice/ iPSC Line (1 clone)Price/ iPSC Line (3 clones)
 Mycoplasma Testing1, 2Please provide the Core with at least 1ml of 2 day old conditioned media. The Core regularly performs mycoplasma testing once a month. Contact the Core for the next available mycoplasma testing date. $17.00$50.00 
Alkaline Phosphatase (AP) Staining1, 2 Please provide the Core with 1 well of 50-60% confluent cells. $40.00$110.00 
Immunocytochemistry (ICC) staining images1, 2
SSEA4 + OCT4  
TRA-1-60 + NANOG
TRA-1-81 + SOX2
Please proved the Core with 1 well of good quality cells that are at least 50-60% confluent.$80.00 $225.00 
 OCT 3/4 + NANOG (or SSEA4) Imaging/Flow Cytometry16 samples, minimum, are required for running this assay. Price may be split between multiple investigators and Core can help coordinate. Please provide the Core with 1 confluent well of each sample.  $120.00 $330.00 
 G-Band Karyotype1, 2Please provide the Core with 2- 80% confluent wells.$450.00 $1250.00 
PluriTest Assay1*Please provide the Core with 1-2- 80% confluent wells.  $320.00  $900.00
 RT-qPCR for endogenous/exogenous
pluripotency gene expression1  
Please provide the Core with 1-2 wells of good quality cells that are at least 80% confluent.  $100.00 $280.00 
 Embryoid Body (EB) formation,
RT-PCR gene expression for 3 lineages1
Please provide the Core with a 70-80% confluent 6-well plate of cells.  $340.00 $950.00 
Teratoma formationPrice per clone. Contact the Core for more information regarding teratoma formation.  $2500.00 $6500.00 
 hPSC Scorecard Assay1*Price is per clone. Please provide the Core with a 70-80% confluent 6-well plate of cells. We will generate EBs and also test parent iPSCs. This assay is done in pair of iPSCs + EBs generated from the iPSCs for 10-18 days. Price reflected is for the pair.  $600.00 $1500.00 
 EBNA1 gDNA PCR1Confirm lack of exogenous plasmid presence. Price is per clone.  $200.00 $550.00 
Southern Blot Analysis   Confirm lack of plasmid integration.$300.00 $850.00 

 Services listed in BOLD are not included on the standard iPSC Generation Package. These services can be requested at an additional charge using the rates described above.

*Only one of either PluriTest or hPSC Scorecard Assay is performed as part of complete characterization suite. Both assays can be performed at additional charge.


 

Bio-Banking and PSC Thawing

As part of the custom iPSC generation service, the core will expand and bank 3 fully characterized iPSC clones per cell line. This service is also available to iPSC and ESC lines generated outside of Cedars-Sinai.

ServiceService DescriptionRequirementsPrice
 Cell Line CryopreservationGuaranteed cryopreservation of high quality, mycoplasma negative cells (6 vials total-feeder free).Please provide the Core with 1 well of starter cells.  $150.00 
 PSC ThawThe Core will thaw 1 vial of PSCs into 1 Matrigel coated well of a 6-well plate and maintain cells for 1 week.  Please provide the Core with 1 vial of cryopreserved cells.  $50.00 
 PSC Thaw and ExpansionThe Core will thaw 1 vial of PSCs into 1 Matrigel coated well of a 6-well plate and expand cells into a full 6-well plate over the course of 2 weeks.   Please provide the Core with 1 vial of cryopreserved cells.$150.00 
 PSC Research Grade Bio-BankThe Core will generate a research grade bank of multiple cryopreserved vials of PSCs.  Karyotype will be performed prior to initiating banking and at the time of creating a research grade lot.  Contact Us


Imaging Services - Image Express Micro, Molecular Devices

ServiceService DescriptionRequirementsPrice
Image Express MicroIndependent acquisition and analysis. User must be trained.  $75.00/hr 
Image Express MicroStaff assisted acquisition and analysis. User must be trained.  $120.00/hr 

 
Cell Lines

To see a complete list of available cell lines, please visit our Stem Cell Lines page. Prices listed reflect academic pricing only. Please contact the iPSC Core for information on industry pricing.

PSC Pricing

ItemVolumeTypePrice*
hiPSCs or hESCs CryovialFrozen $650.00 
hiPSCs or hESCs*  6-well plate Live Culture $120.00 ($20.00/well) + $650.00 (cost of vial) = $770.00 
Reporter cell lines (iPSCs or ESCs) Nuclear GFP in AAVS1 safe harbor locus6-well plate Live Culture $120.00 
 Reporter cell lines (iPSCs or ESCs) Nuclear GFP in AAVS1 safe harbor locus CryovialFrozen $700.00 

MEF Pricing

PSC qualified and mitomycin-C treated

ItemVolumeTypePrice*
MEF vial suitable for 3 plates Cryovial - 5x10^6 cells  Frozen $70.00 
MEF vial suitable for 6 plates Cryovial - 10x10^6 cells  Frozen $130.00
Mouse Embryonic Fibroblasts (MEF) 6-well plate Live culture $30.00 
Mouse Embryonic Fibroblasts (MEF)T75 flask Live culture $35.00

*Shipping and Tax will be additional

Custom Mouse Embryonic Fibroblast (MEF) Generation

Contact the Core for custom generation of P1 and P2 non-mitomycin treated MEFs.

Training

The iPSC Core trains graduate students, postdoctoral fellows and technicians in stem cell biology techniques. To schedule a training appointment, please contact the iPSC Core.