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There are five areas of study in the Yang Laboratory.
The Yang Laboratory has developed a powerful palmitoyl-proteomics method termed Palmitoyl Protein Identification and Site Characterization. Currently, we focus on identifying substrate proteins and sites of depalmitoylating enzymes that play critical roles in prostate cancer metastasis and castration resistance.
The Yang Lab conducts quantitative comparison of thousands of proteins across different samples. Most commonly used techniques include tandem mass tags, stable-isotope labeling by amino acids in cell culture and label-free quantification using MaxLFQ and spectral counting.
We apply whole proteome sequencing to determine the copy numbers of more than 10,000 expressed proteins, and to identify protein products of point mutations, unusual splice variants, 5'-UTR translation and gene fusions.
The Yang Laboratory focuses on identifying key kinases involved in drug resistance and DNA damage response, by label-free quantitative phosphoproteomics.
Crosslinking Mass Spectrometry
The Yang Lab applies crosslinking mass spectrometry to reveal the architecture of protein complexes (e.g., the uroplakin complex).